Biotin repressor stress

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August undefined, 2024

WebNov 4, 2005 · The co-repressor buttresses the dimer interface, resulting in improved packing and a 12 degrees change in the hinge-bending angle along the dimer interface relative to the BirA.biotin structure. This helps explain why the binding of the co-repressor is necessary to optimize the binding of BirA to the bioO operator. WebNov 14, 2024 · In this study, we establish OmpR as a global regulator of acid and osmotic stress in S. Typhimurium and E. coli. OmpR represses distinct sets of genes based on the intracellular pH threshold...

The biotin repressor: modulation of allostery by corepressor analo…

WebFeb 1, 1999 · Abstract. Enzymatic attachment of biotin to proteins requires the … WebThe identity of the biotin repressor and the biotin-apo- BCCP ligase of E. coli was strongly indicated by the very detailed genetic studies of Barker and Campbell (3, 4) who showed that lesions in the same genetic entity, the birA gene, gave rise to both biotin-apoBCCP ligase deficiency and to depression of the biotin operon. green face shield

Functional definition of BirA suggests a biotin …

WebMar 24, 2006 · BirA is a bifunctional enzyme that adenylates biotin and then uses the … WebSep 24, 1999 · The Escherichia coli repressor of biotin biosynthesis (BirA) is an allosteric site-specific DNA-binding protein. BirA catalyzes synthesis of biotinyl-5′-AMP from substrates biotin and ATP and the adenylate serves as the positive allosteric effector in binding of the repressor to the biotin operator sequence. Web2 days ago · We found that the expression of genes related to a lytic phage lifestyle in the phage-plasmid were highly up-regulated after the observation of mutations in the repressor sequence, such as the... green flower sweater

Molecular Biology of Biotin Attachment to Proteins

4WF2: Structure of E. coli BirA G142A bound to biotinol-5

WebNov 11, 1991 · Proteins most abundant in S-phase cells were stress associated and transporters plus transferases in agreement with the general phenomenon that binding protein-dependent systems are induced under nutrient limitation as part of hunger response. ... The Escherichia coli biotin repressor is a member of the “winged helix-turn-helix” … green flag directionsWebNov 14, 2024 · Acid stress reduces the internal pH below this threshold, increasing OmpR dimerization and DNA binding. ... in the ompR null mutant, suggesting that E. coli OmpR also functions as a repressor at ... green electronic solutions dallas

"WebFeb 13, 1996 · The Escherichia coli repressor of biotin biosynthesis (BirA) is an allosteric site-specific DNA binding protein. The protein is composed of three structural domains. Contact with the biotin operator (bioO) in the transcriptional repression complex is made by the N-terminal domain which contains a helix−turn−helix structural module. The … " - Biotin repressor stress

Biotin repressor stress

Coupling of Protein Assembly and DNA Binding: Biotin Repressor ...

WebBiotin is a member of the vitamin B-complex family. Biotin deficiency has been … WebJan 31, 2003 · The biotin repressor is an allosterically regulated, site-specific DNA-binding protein. Binding of the small ligand bio-5′-AMP activates repressor dimerization, which is a prerequisite to DNA binding. Multiple disorder-to-order transitions, some of which are known to be important for the functional allosteric response, occur in the vicinity ...

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WebNational Center for Biotechnology Information WebMay 22, 2001 · The Escherichia coli biotin repressor binds to the biotin operator to …

WebJan 1, 2001 · Cooperative association of the Escherichia coli biotin repressor with the biotin operator is allosterically activated by binding of the corepressor, bio-5'-AMP. The corepressor function of... WebNov 9, 2002 · The Escherichia coli biotin repressor, an allosteric transcriptional regulator, is activated for binding to the biotin operator by the small molecule biotinyl-5‘-AMP. Results of combined thermodynamic, kinetic, and structural studies of the protein have revealed that corepressor binding results in disorder to order transitions in the protein monomer that …

WebJan 12, 2001 · The Escherichia coli biotin repressor binds to the biotin operator to repress transcription of the biotin biosynthetic operon. In this work, a structure determined by x-ray crystallography of a complex of the repressor bound to biotin, which also functions as an activator of DNA binding by the biotin repressor (BirA), is described ... WebMar 5, 2024 · BirA is a transcriptional repressor of the biotin biosynthesis operon ( bioO, shaded gray) in both bacteria. BirA is proposed to also regulate expression of the biotin transport protein BioY and the yhfS - yhfT operon in S. aureus. b Sequences of the promoters for bioO in E. coli and bioO, bioY, and yhfS-yhfT in S. aureus are shown.

WebMay 15, 2001 · The biotin repressor, BirA, is a critical protein in regulation of biotin retention and biosynthesis in Escherichia coli.Biotin is retained in the cell as a coenzyme that is covalently linked to biotin-dependent carboxylases ().In E. coli this two-step …

WebEnzymatic Biotinylation: BirA. Ligation of biotin to a protein is a specific post-translational … green fox training studioWebSep 11, 2014 · Small ligand, bio-5'-AMP, binding and dimerization of the Escherichia coli biotin repressor are allosterically coupled ... Macromolecules Find similar proteins by: (by identity cutoff) 3D Structure Small Molecules Experimental Data & Validation Experimental Data Method: X-RAY DIFFRACTION Resolution: 2.31 Å R-Value Free: 0.218 R-Value … green fabric storage binsWebNov 12, 2024 · OsClo5 functions as a transcriptional co-repressor by interacting with OsDi19-5 to negatively affect salt stress tolerance in rice seedlings. Pei Jing, ... The MBP-OsDi19-5 was incubated with the biotin-labeled wild-type Di19-binding sequence (DIBS) probe or mutated DIBS probe, and the non-labeled DIBS probe was used as a competitor ... green forest print fabricWebFeb 1, 2003 · The biotin repressor is an allosterically regulated, site-specific DNA-binding protein. Binding of the small ligand bio-5'-AMP activates repressor dimerization, which is a prerequisite to DNA binding. green from rainbow friends in robloxWebSep 25, 2001 · Diphtheria toxin repressor protein (DtxR) is a 226-amino acid residue, iron-dependent transcriptional regulator of genes in Corynebacterium diphtheriae that code for diphtheria toxin and proteins involved in iron uptake and oxidative stress (1, 2).DtxR is the best characterized member of a family of related proteins from primarily Gram-positive … green giant privacy treesWebDec 25, 1982 · A bifunctional protein. Definitive evidence is presented for the bifunctional … green glass textureWebMar 5, 2024 · Biotin biology in E. coli and S. aureus.a BirA is a multi-functional protein … green grass of wyoming o\u0027hara