Thp-1 cells คือ
Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their … See more THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and See more • Cellosaurus entry for THP-1 See more WebOct 7, 2024 · THP-1 cells (ATCC® TIB202™) were cultured using RPMI-1640 (Life Technologies) supplemented with 2 mM L-glutamine, 10% FCS and Penstrep (100 U/ml) at 37°C, 5% CO 2.Cells were grown to a density of 5 × 10 5 –1 × 10 6 cells/ml and used for experiments between passage 5 and 12. For THP-1 compound treatment and …
Thp-1 cells คือ
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WebA potential trajectory is to use this cell line to study the novel molecular mechanisms in monocytes and macrophages in relation to the physiology and pathophysiology of the … WebInvivoGen’s THP-1 cells also respond to NOD1, RIG-I, MDA-5 and STING/cGAS agonists. ... (PMA, catalog #tlrl-pma) induces differentiation of THP1 cells into adherent macrophage-like cells. Day 1 1- Add 180 µl of THP1 cell suspension per well of a 96-well plate (~ 100 000 cells/well). 2- Treat THP1 cells with 20 µl of PMA (final concentration ...
WebThe human monocytic cell line, THP-1, is the most widely used model for primary human monocytes/macrophages. This is because, following differentiation using phorbol 12-myristate 13-acetate (PMA), THP-1 cells acquire a macrophage-like phenotype, which mimics, in many respects, primary human macropha … Web3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take at …
Web12 Transfer cells + media to a T-25 flask 13 Incubate cells at 37 °C and 5% CO2 and 80% humidity 14 THP-1 cells replicate after ~26 hours. In practice, it takes 2 days for a true … WebCytogenetic and molecular analysis of the acute monocytic leukemia cell line THP-1 with an MLL-AF9 translocation. Genes Chromosomes Cancer 29:333-338(2000) DOI= …
WebMay 25, 2024 · A previous study showed that the expression of pro-inflammatory cytokines differs between THP-1 cells and PMA-differentiated THP-1 cells. 11 To further investigate whether these differences depend on the LPS concentration in THP-1 cells and PMA-differentiated THP-1 cells, the expression levels of mRNA and protein were analyzed …
WebAug 14, 2014 · Besides that an overview of newly released commercial THP-1 engineered-reporter cells and THP-1 inflammasome test-cells is also given. Evaluation of recent papers leads to the conclusion that the THP-1 cell line has unique characteristics as a model to investigate/estimate immune-modulating effects of compounds in both activated and … hogarth agenciaWebTHP1-defASC cells are derived from THP-1 human monocytic cells. THP-1 cells are the most commonly used model cell line for the study of inflammasome activation as they express high levels of NLRP3, ASC and pro-caspase-1. THP1-defASC cells express negligible levels of ASC but express native levels of NLRP3 and pro-caspase-1. These cells are ... hogarth agency londonWebDec 30, 2024 · Good day, I am having problems with culturing my THP-1 cells. I tried culturing them in several different volumes, I was recommended to seed 5,000,000 cells in 100mL to 150mL and let them sit ... hogarth analysis of beautyWeb1. To revive THP-1 and Jurkat cells from frozen liquid N 2 stocks, warm RPMI-1640 supplemented with FBS (10%, complete media) to 37 °C in a water bath.. 2. Thaw the capped frozen vial containing cells in a water bath at 37 °C.Note: Only a partial thaw is sufficient, and a small lump of ice should remain in the vial. Limit the toxicity of the DMSO (5%) … hub 3 flashing green arrowsWebJan 12, 2024 · Once the cells are suspended, seal the tube, invert a few times, and then divide the cells into the freezing vials. Transfer the vials to your equipment for freezing. Do Not Leave Your THP-1 Cells in the -80°C … hogarth advertising londonWebNov 1, 2014 · The THP-1 cell line is isolated from the peripheral blood of a 1-year old male patient suffering from acute monocytic leukemia [7]. This cell line has been widely used to study immune responses while cells are not only in the monocyte state but also in the macrophage-like state [8], [9]. THP-1 macrophages, when stimulated with LPS, expressed ... hogarth accent cabinethub 3d printing